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BFS-011 · Experimental Feed Science · May 2026
⚗ EXPERIMENTAL — Cycle 1 Box Trial

Experimental Feed Additive Stack

Source: SBS Philippines (sbsph.com) raw material catalog — Animal Health & Nutrition line

Farm: Paombong, Bulacan · 6 ha brackishwater ponds · Box 1 (experimental) vs Box 2 (control)

Existing pipeline: Probiotics (BFS-009) · Phytase + High-Protein Starter (BFS-010)

Goal: Identify additional raw materials from SBS PH that can further improve FCR and growth rate, without requiring a feed pelleter — applied as pellet-coating on commercial base feed

Table of Contents

  1. Approach — Coating vs DIY Pelleting
  2. ⚗ NEW: Betaine — The Euryhaline Advantage
  3. Compound Enzyme Blend (Upgrade from Phytase Alone)
  4. Lysine HCL — #1 Limiting Amino Acid
  5. Choline Chloride — Grow-Out Stage
  6. Full Stack by Stage
  7. Application Method — No Equipment Needed
  8. Trial Design: Box 1 vs Box 2
  9. What to Skip from the SBS Catalog
  10. Cost Estimate

Approach — Coating vs DIY Pelleting

Making feed pellets from scratch requires a pelleting extruder (₱50–150k), binder control, moisture management, and particle-size consistency. At your stocking density (~13,500/ha), lablab and phytoplankton still supply a significant portion of nutrition in the first 60 days — commercial feed is supplemental, not the sole diet. DIY pellets that break apart in water before the fish eat them are worse than a commercial pellet with additives coated on.

Better approach: Buy Tateh, Grobest, or Vitarich as the base pellet. Mix and coat specific SBS raw material additives directly onto the pellets before each feeding. You get full control over the high-impact ingredients without any equipment investment. This is how commercial premix programs work — the additive stack rides on top of a quality base feed.
DIY Pelleter Cost
₱50–150k
not needed with coating
Coating Equipment
₱0
bucket + scale + mixing
Additive Stack Cost
~₱5–12k
per box per cycle (est.)
Complexity
Low
Rain can execute daily

⚗ NEW — Betaine: The Euryhaline Advantage

⚗ NEW — Not Yet in Pipeline

Betaine (Trimethylglycine)

SBS PH product: Listed under Amino Acids. Form: powder or liquid concentrate.

Dose: 0.5–1% of total feed weight per feeding

Stage: Both fingerling and grow-out

Why This Is the Most Underutilized Lever for Milkfish Specifically

Milkfish (Chanos chanos) is a naturally euryhaline species — it evolved moving between seawater, estuaries, and brackishwater. In your Paombong ponds, salinity fluctuates significantly during the June–October monsoon season (can swing from 30 ppt down to 5–10 ppt during heavy rain events). Every time salinity changes, the fish must expend metabolic energy on osmoregulation — adjusting intracellular ion concentrations to match the external environment.

Betaine is an organic osmolyte (compatible solute). When fish absorb betaine from feed, they can use it directly as an intracellular osmolyte, reducing the energy cost of biosynthesizing their own osmotic regulators. The energy saved is redirected into growth. This is mechanistically specific to euryhaline and marine fish — it is not a general fish supplement. Milkfish is one of the ideal candidates.

Evidence Base

Study / SourceSpeciesFinding
Xue et al. (2006) — AquacultureJapanese flounder (euryhaline marine)Betaine at 1% diet improved FCR by 12%, weight gain by 15% under salinity stress
Gaylord et al. (2006) — Aquaculture NutritionRainbow troutBetaine at 1% spared methionine, improved protein retention efficiency 11%
Wang et al. (2021) — AquacultureTurbot (marine)Betaine improved growth, reduced cortisol stress markers during salinity challenge
SEAFDEC/AQD feed additive reviewMilkfish (general)Listed as an "effective feed attractant and growth promoter" in milkfish starter diets — appetite stimulation secondary mechanism

Bonus mechanism: Betaine is also a potent feed attractant — it increases the palatability and feed intake rate of fish. In ponds where natural food is competing with commercial pellets, betaine coating on pellets makes the commercial feed more attractive, improving actual feed uptake per feeding event.

Numbers Estimate for Your Farm

Without BetaineWith Betaine (conservative 10% FCR gain)With Betaine (optimistic 15% gain)
FCR (post-phytase baseline)1.601.441.36
Feed cost saved/box/cycle~₱48,000~₱72,000
Betaine cost/box/cycle~₱2,000–3,000~₱2,000–3,000
ROI on betaine cost16–24×24–36×

Compound Enzyme Blend (Upgrade from Phytase Alone)

Upgrade — Replaces Standalone Phytase

Compound Enzyme Blend: Phytase + Protease + Xylanase

SBS PH product: Listed under Enzymes → "Compound Blends"

Dose: Per label (typically 0.05–0.1% of feed, or 500–1,000g per tonne of feed)

Stage: Fingerling and early grow-out (Days 1–90)

Why the Blend Outperforms Phytase Alone

Phytase — breaks down phytic acid → releases bound phosphorus + improves protein digestibility. Already proven in milkfish (Philippine Journal of Science). FCR improvement: 10–20%.

Protease — directly breaks down feed proteins into amino acids. Adds another 10–15% improvement in protein digestibility on top of phytase. Works on both plant and animal protein sources.

Xylanase — breaks down non-starch polysaccharides (NSPs) in rice bran and soy. NSPs are the "cage" that traps nutrients inside plant cell walls. Xylanase unlocks them.

EnzymeFCR ImprovementStandalone
Phytase only+10–20%Current plan
Protease added+additional 10–15%
Xylanase added+additional 5–8%
Full blend+25–40% total FCR gainRecommended

Combined enzyme effects are additive, not exponential. Actual gain depends on feed composition — higher plant-based ingredient content = greater enzyme benefit.

Action: Ask SBS PH for their compound enzyme blend SKU. If they can supply phytase + protease + xylanase in one premix, buy that instead of standalone phytase. Same application method, better result, one product to manage.

Lysine HCL — #1 Limiting Amino Acid

GO — Fingerling Stage Priority

Lysine HCL (L-Lysine Monohydrochloride)

SBS PH product: Listed under Amino Acids → Lysine HCL

Dose: 0.3–0.5% of feed weight (supplement on top of commercial feed protein)

Stage: Fingerling (Days 1–60). Taper at grow-out if using high-CP commercial feed.

Why Lysine Is the Critical Gap in Plant-Based Feeds

Plant-based feed ingredients (rice bran, soybean meal, corn) are all deficient in lysine relative to what fish require for optimal protein synthesis. When a fish's diet is lysine-deficient, it cannot efficiently use the protein in the feed — amino acids are burned for energy instead of being deposited as muscle.

Supplementing lysine directly closes this gap and forces more dietary protein into growth rather than catabolism. This is especially important during the fingerling stage (Days 1–60) when you're feeding the high-protein 28–32% CP starter and the fish are in their fastest growth phase.

SEAFDEC/AQD Milkfish Nutrition Review — Lysine is identified as the primary limiting amino acid in plant-protein-based milkfish diets. Requirement: approximately 1.5–2.0% of diet on a dry matter basis. Most commercial bangus feeds target this range, but supplementation provides a buffer when lablab (variable protein quality) is a major food source in the first 60 days.
ScenarioLysine SupplementedExpected Effect
Fingerling + commercial 28% CP + lablabYes, 0.3% supplementProtein efficiency improves — more feed protein becomes body protein
Grow-out + standard commercial 22% CPOptional, 0.2%Smaller benefit if commercial feed is already adequately formulated
Fingerling + rice bran supplement onlyYes — criticalWithout lysine, plant-based supplement protein is mostly wasted as energy

Choline Chloride — Grow-Out Stage

Grow-Out Stage · Liver Protection

Choline Chloride 60%

SBS PH product: Listed under Feed Additives & Minerals → Choline Chloride

Dose: 0.05–0.1% of feed (500–1,000 mg/kg feed)

Stage: Grow-out (Days 60–harvest). Less critical at fingerling if using a complete commercial feed.

Why It Matters at Grow-Out

Choline is essential for fat metabolism — specifically for transporting lipids out of the liver. Without adequate choline, fat accumulates in the liver (hepatic lipidosis / "fatty liver"), which impairs liver function, suppresses immunity, and ultimately reduces growth rate as the fish's metabolism becomes less efficient.

As your fish accumulate body mass toward 300–400g at harvest, fat deposition increases and choline demand rises. Cheap supplemental feeds (rice bran, trash fish) are typically low in choline. Commercial pellets usually include it, but at minimum levels. Adding a small supplement is inexpensive insurance.

Practical note: If you're using premium commercial pellets (Grobest, Vitarich) throughout grow-out, choline is likely already adequate. If you're transitioning to lower-cost supplemental feeds (rice bran) at grow-out to reduce cost, add choline chloride at 0.05–0.1% of the rice bran supplemental ration.

Full Additive Stack by Stage

StageDaysAdditiveDosePurposeSource
Fingerling 1–60 High-Protein Base Feed (28–32% CP) As directed SEAFDEC 2025 protocol — core of 85-day grow-out Grobest / Vitarich starter
Betaine 0.5–1% of feed weight Osmoprotection → energy to growth; feed attractant SBS PH — Amino Acids
Compound Enzyme Blend Per label (~0.05–0.1%) Phytase + Protease + Xylanase — full digestibility upgrade SBS PH — Enzyme Blends
Lysine HCL 0.3–0.5% of feed weight Close the #1 limiting amino acid gap in plant-based feeds SBS PH — Amino Acids
Grow-Out 60–harvest Standard commercial feed (22–25% CP) or rice bran supplement 4% body weight/day Transition from high-CP starter Tateh / Feedmix / Grobest
Betaine 0.5% of feed weight Continue osmoprotection through harvest SBS PH — Amino Acids
Compound Enzyme Blend Per label (half dose) Maintain digestibility improvement at lower level SBS PH — Enzyme Blends
Choline Chloride 0.05–0.1% of supplemental feed Prevent fatty liver accumulation as fish approach market weight SBS PH — Feed Additives

Existing pipeline continues unchanged: Probiotics (UPLB BIOTECH MicroBead or OGANIKKU) applied per BFS-009 caretaker protocol. This stack adds on top — it does not replace probiotics.

Application Method — No Equipment Needed

All additives are applied as a pellet coating, not mixed into a new feed formulation. Rain (caretaker) can execute this with a bucket, kitchen scale, and mixing spoon.

Step 1 — Weigh the day's feed ration
Morning, before each feeding
Determine kg of commercial pellets for each feeding session. Typical: 2% body weight AM + 2% body weight PM (adjust as fish grow and you estimate total biomass).
Step 2 — Dissolve liquid additives first
Betaine + any liquid probiotics
If betaine is in powder form, dissolve in 50–100ml of water per kg of feed. If liquid form, add directly. Mix into pellets and let absorb for 5 minutes. Pellets will become slightly damp — this is normal and helps enzyme adhesion in the next step.
Step 3 — Add dry powders last
Enzyme blend + Lysine HCL + Choline Chloride
Add all dry powder additives after the liquid absorption step. Mix thoroughly to coat pellets. Enzyme activity degrades in water — always add dry and always feed within 20 minutes of mixing. Do NOT pre-batch large quantities in advance.
Step 4 — Apply to pond within 20 minutes
Broadcast or use feeding trays
Feed as normal. If you are doing 3 feedings/day (BFS-010 recommendation), prepare each batch separately. Do not mix the full day's ration at once in the morning.
Record keeping: Rain should log every feeding with: date, time, kg of feed, kg of each additive used. This is the only way to know if the trial data is valid at harvest. Use the farm log sheet from BFS-001 Operations Manual.

Trial Design — Box 1 (Experimental) vs Box 2 (Control)

Box 1 — Full Experimental Stack

  • Probiotics (existing — BFS-009)
  • High-Protein Starter Feed 28–32% CP (Days 1–30)
  • Betaine 0.5–1% (new)
  • Compound Enzyme Blend — phytase + protease + xylanase (upgrade)
  • Lysine HCL 0.3–0.5% (new)
  • Choline Chloride 0.05% at grow-out (new)
  • Third daily feeding if labor permits
  • Partial harvest at Day 40–60

Box 2 — Control (Current Pipeline Only)

  • Probiotics (existing — BFS-009)
  • Standard commercial feed (Tateh / Grobest)
  • Phytase standalone (BFS-010)
  • Standard 2× daily feeding
  • No betaine, lysine, choline supplement
  • Standard single harvest at end of cycle

What to Measure at Harvest

MetricBox 1 TargetBox 2 BaselineHow to Measure
Days to harvest<90 days~110 daysCount from stocking date
Average body weight (ABW) at harvest>350g~300–350gSample 30 fish, weigh, average
Survival rate>80%~75–80%(Fish harvested ÷ fish stocked) × 100
Total harvest weight (kg)>12,000 kg~9,000–10,000 kgWeigh at buyout
FCR<1.4~1.6–1.8Total feed used (kg) ÷ total fish weight gained (kg)
Net profit>₱1.1M~₱850kRevenue − all costs
Decision rule: If Box 1 FCR beats Box 2 by ≥0.2 points OR harvest is ≥10 days shorter, apply the full experimental stack to both boxes from Cycle 2. If results are within margin of error (less than 5% difference), investigate which additive to remove and re-test Cycle 2 with a reduced stack.

What to Skip from the SBS Catalog

ProductReason to Skip
Beta CaroteneImproves pigmentation and visual appearance — not a growth driver. Relevant for ornamental fish, not market bangus.
Glucosamine Sulfate / Chondroitin SulfateJoint supplements. Relevant for land animals. No established benefit for milkfish grow-out.
Chromium PicolinateInsulin-sensitizing trace mineral. Negligible impact at milkfish grow-out densities.
B-complex vitamins (B1, B2, B3, B12)Already included at adequate levels in any commercial pellet. Don't add cost without a known deficiency.
Vitamin A (multiple forms)Same — adequately covered by commercial feed. Toxicity risk at over-supplementation.
Copper Sulphate, Zinc OxideTrace mineral supplements — adequate in commercial feeds. Zinc oxide at high doses can be toxic in aquatic environments.
Soy Protein Concentrate, Fish Meal, Fish OilBulk ingredients for DIY pelleting, not additive coating. Only relevant if you later build a custom feed formulation at large scale.
Taurine, Histidine, Isoleucine, Valine, Glycine, Cysteine, Arginine, Threonine, TryptophanSecondary or tertiary limiting amino acids in milkfish diets. Lysine and methionine come first. Adding all amino acids simultaneously is overkill and creates imbalance — start with lysine only.

Cost Estimate

AdditiveDoseEstimated Qty/box/cycleEstimated Cost/box/cycle
Betaine (powder)0.5–1% of feed90–180 kg over cycle₱2,000–4,000
Compound Enzyme Blend0.05–0.1% of feed9–18 kg over cycle₱1,500–3,000
Lysine HCL0.3–0.5% of feed (Days 1–60 only)5–10 kg₱800–1,500
Choline Chloride0.05–0.1% (grow-out only)3–6 kg₱400–800
Total additive stack₱4,700–9,300 per box per cycle
Feed cost saved at conservative 20% FCR improvement₱60,000–80,000 per box per cycle
Net gain after additive cost₱50,000–75,000 per box per cycle
ROI on the full experimental stack: ~8–12× per cycle, per box. At two boxes, the stack pays for itself within the first month of the first cycle if FCR improves by even 15%. Contact SBS PH directly to request pricing and minimum order quantities before July 3 stocking.

Contact SBS Philippines

Website: sbsph.com — Animal Health & Nutrition

Request: pricing for Betaine, Compound Enzyme Blend (phytase+protease+xylanase), Lysine HCL, and Choline Chloride 60% — minimum order quantities and aquaculture-grade specs.

BFS-011 · Experimental Feed Additive Stack · Paombong, Bulacan · May 2026
Source: SBS Philippines (sbsph.com) Animal Health & Nutrition catalog